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Hydrophilic interaction chromatography coupled to MS for metabonomic/metabolomic studies
Author(s) -
Spagou Konstantina,
Tsoukali Helen,
Raikos Nikolaos,
Gika Helen,
Wilson Ian D.,
Theodoridis Georgios
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900803
Subject(s) - hydrophilic interaction chromatography , metabolomics , chemistry , metabolome , chromatography , metabolite , analyte , reversed phase chromatography , high performance liquid chromatography , biochemistry
Hydrophilic interaction chromatography (HILIC) is a relatively recently introduced mode of liquid‐phase separations. Recently, HILIC has been used for coupling to MS in metabonomic/metabolomic studies to provide a complementary tool to the widely used reversed‐phase (RP) chromatographic separations. The combination of HILIC with MS detection covers a number of polar metabolites that are typically nonretained in RPLC‐mode separations and thus enlarging the number of detected analytes. This way of metabolite profiling thus provides more comprehensive metabolome coverage than using RP chromatography alone. This review describes the applications and the utility of HILIC‐MS in metabolomic/metabonomic studies and highlights certain characteristic examples in the life and plant‐food sciences.