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Application of turbulent flow chromatography to the metabonomic analysis of human plasma: Comparison with protein precipitation
Author(s) -
Michopoulos Filippos,
Edge Antony M.,
Theodoridis Georgios,
Wilson Ian D.
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900789
Subject(s) - chromatography , protein precipitation , chemistry , metabolite , human plasma , precipitation , methanol , high performance liquid chromatography , sample preparation , solvent , biochemistry , organic chemistry , physics , meteorology
The use of turbulent flow chromatography (TFC) as a method for the rapid metabonomic LC‐MS analysis of plasma as an alternative to solvent‐based protein precipitation has been investigated. This comparison has shown that TFC can be effectively used in this application with the benefit that off‐line sample handling is significantly reduced. However, analysis of the data obtained via TFC for human plasma reveals substantial differences in the overall metabolite profiles compared with methanol‐precipitated HPLC‐MS. This seems in part at least to be related to greatly reduced amounts of phospholipids (ca. 10 fold reduction) for the turbulent flow methodology compared with protein‐precipitated samples. The significance of these differences with respect to metabolite profiles as a result of the sample preparation method used are discussed.