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Preparative separation of major xanthones from mangosteen pericarp using high‐performance centrifugal partition chromatography
Author(s) -
Shan Yichu,
Zhang Wei
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900774
Subject(s) - chemistry , chromatography , ethyl acetate , petroleum ether , sephadex , silica gel , extraction (chemistry) , solvent , partition coefficient , high performance liquid chromatography , methanol , countercurrent chromatography , organic chemistry , enzyme
Mangosteen fruit pericarp (MFP) is a rich source of xanthones, which has shown remarkable pharmacological activities. To isolate xanthones, previous methods included labor intensive and time‐consuming solid‐phase extractions (Sephadex LH20, silica gel) and sequential solvent extraction. In this study, major xanthones (α‐ and γ‐mangostins) in MFP were isolated at high purity in one step utilizing high‐performance centrifugal partition chromatography with solvent system composed of petroleum ether, ethyl acetate, methanol and water (10:5:5:1). In one run, 200 mg crude extract of MFP was injected and 55.4 mg α‐mangostin and 12.4 mg γ‐mangostin were obtained with the purity of 93.6 and 98.4%, respectively. The yields of them were 86.3 and 76.3%, respectively. As α‐ and γ‐mangostins are reported to show potent antioxidant, anti‐inflammatory and anticancer activities, this method can be used for the large‐scale production of them for future in vitro and in vivo biological studies.