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Separation and quantitative determination of sesquiterpene lactones in Lindera aggregata (Wu‐yao) by ultra‐performance LC‐MS/MS
Author(s) -
Wu Yongjiang,
Zheng Yunliang,
Liu Xuesong,
Han Zheng,
Ren Yiping,
Gan Lishe,
Zhou Changxin,
Luan Lianjun
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900768
Subject(s) - chromatography , formic acid , chemistry , lauraceae , sesquiterpene , gradient elution , high performance liquid chromatography , sesquiterpene lactone , stereochemistry , botany , biology
An ultra‐performance LC in combination with MS/MS method was established to evaluate the quality of Wu‐yao (the dried root of Lindera aggregata (Sims) Kosterm. (Lauraceae)) through simultaneous quantitation of five sesquiterpene lactones, linderagalactone D ( 1 ), linderagalactone C ( 2 ), hydroylindestenolide ( 3 ), neolinderalactone ( 4 ) and linderane ( 5 ). All compounds were separated on a Waters Acquity ultra‐performance LC HSS T3 column (2.1 mm×100 mm, 1.8 μm particle size) with linear gradient elution of acetonitrile and 0.1% formic acid. An ESI interface in the positive mode was employed prior to MS detection. All the compounds showed good linearity ( R 2 ≥0.9992). The recoveries, measured at three concentration levels, varied from 97.3 to 103.4% with RSDs <4.8%. The simple, rapid and reliable method was successfully applied to quantify the five components in 13 samples of Wu‐yao from different areas.