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Preparative isolation and purification of anti‐tumor agent ansamitocin P‐3 from fermentation broth of Actinosynnema pretiosum using high‐performance counter‐current chromatography
Author(s) -
Yao Yuqin,
Cheng Zhihui,
Ye Haoyu,
Xie Yongmei,
He Jing,
Tang Minghai,
Shen Tao,
Wang Jiangman,
Zhou Yan,
Lu Zejun,
Luo Feng,
Chen Lijuan,
Yu Luoting,
Yang JinLiang,
Peng Aihua,
Wei Yuquan
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900746
Subject(s) - chromatography , fermentation , chemistry , high performance liquid chromatography , ethyl acetate , solvent , mass spectrometry , organic chemistry
Ansamitocin P‐3 is a potent anti‐tumor maytansinoid found in Actinosynnema pretiosum . However, due to the complexity of the fermentation broth of Actinomycete, how to effectively separate ansamitocin P‐3 is still a challenge. In this study, both analytical and preparative high‐performance counter‐current chromatography were successfully used to separate and purify ansamitocin P‐3 from fermentation broth. A total of 28.8 mg ansamitocin P‐3 with purity of 98.4% was separated from 160 mg crude sample of fermentation broth in less than 80 min with the two‐phase solvent system of hexane–ethyl acetate–methanol–water (0.6:1:0.6:1, v/v/v/v). The purity and structural identification were determined by HPLC, 1 H NMR, 13 C NMR and mass spectroscopy.