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Single‐step separation of bioactive flavonol glucosides from Osteomeles schwerinae by high‐speed counter‐current chromatography
Author(s) -
Lee Jun,
Jang Dae Sik,
Yoo Nam Hee,
Lee Yun Mi,
Kim JooHwan,
Kim Jin Sook
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900693
Subject(s) - chromatography , countercurrent chromatography , chemistry , high performance liquid chromatography
Abstract Two bioactive flavonol glucosides, hyperoside and quercitrin, were successfully isolated in one step from the phytochemically unknown medicinal plant Osteomeles schwerinae by high‐speed counter‐current chromatography using a two‐phase solvent system composed of n ‐hexane–ethyl acetate–methanol–water (0.5:5.5:1.5:4.5 v/v). From 160 mg of crude extract, 2.1 mg of hyperoside (98.6% purity, 83.3% recovery) and 4.5 mg of quercitrin (99.2% purity, 81.7% recovery) were separated and then their chemical structures were identified by 1 H and 13 C NMR analysis. In addition, the isolates were subjected to an in vitro bioassay to evaluate their inhibitory activity on rat lens aldose reductase. Both quercitrin and hyperoside showed excellent inhibitory activities toward rat lens aldose reductase with the IC 50 values of 0.16 and 4.33 μM, respectively, as compared with positive control, 3,3‐tetramethyleneglutaric acid (28.7 μM). So far, chemical constituents and biological activities of O. schwerinae have never been reported. This is the first report on the chemical constituents and biological activity of this plant using semi‐preparative high‐speed counter‐current chromatography separation technique.