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Simultaneous determination of seven main alkaloids of Chelidonium majus L. by ultra‐performance LC with photodiode‐array detection
Author(s) -
Gu Yue,
Qian Dawei,
Duan Jinao,
Wang Zhenzhong,
Guo Jianming,
Tang Yuping,
Guo Sheng
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900690
Subject(s) - chelidonium , coptisine , protopine , sanguinarine , chromatography , repeatability , chelerythrine , chemistry , ammonium acetate , analyte , photodiode , acetic acid , high performance liquid chromatography , alkaloid , analytical chemistry (journal) , palmatine , materials science , stereochemistry , organic chemistry , optoelectronics , protein kinase c , horticulture , biology , enzyme
A simple and rapid method for the simultaneous determination of seven isoquinoline alkaloids, protopine, chelidonine, coptisine, stylopine, sanguinarine, berberine, and chelerythrine, in Chelidonium majus L. ( Ch. majus ) samples by ultra‐performance LC method with photodiode array detection is described. The baseline separation of these compounds was performed with (A) acetonitrile–(B) ammonium acetate (10 mM, adjusted to pH 3.0 with acetic acid) as the mobile phase using a C18 RP column (2.1×100 mm, 1.7 μm). Optimized conditions resulted in excellent peak shapes. The seven alkaloids were completely separated within 20 min. Good linear behaviors ( r ≥0.9992) over the investigated concentration ranges were observed for all the analytes. Validation proved the repeatability of the method was good and recovery was satisfactory. The validated method was successfully applied for 20 batches of Ch. majus . These results demonstrated that the ultra‐performance LC photodiode array method proposed was very useful in the analysis and quality control of Ch. majus .