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Separation of α‐ from β‐arylalanines by nickel nitrilotriacetate chromatography
Author(s) -
Wijeratne Salinda,
Byrne Noelle A.,
Walker Kevin D.
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900675
Subject(s) - chemistry , chromatography , elution , agarose , resolution (logic) , nickel , two dimensional chromatography , matrix (chemical analysis) , fraction (chemistry) , ligand (biochemistry) , high performance liquid chromatography , organic chemistry , biochemistry , receptor , artificial intelligence , computer science
A method is described to separate α‐ from β‐arylalanines by ligand exchange chromatography on a nickel nitrilotriacetate agarose column with UV monitoring of the effluent. Separate mixtures containing an α‐ and β‐arylalanine pair (1 mg of each) were individually loaded onto the nickel resin pre‐equilibrated with the mobile phase at room temperature, and the amino acids were eluted from the column with a gradient from pH 12.0–8.0. The β‐arylalanines eluted first, followed by the α‐isomers. The four α/β‐amino acid pairs tested were well separated with baseline resolution. An aliquot of each fraction was chemically treated to derivatize the amino acids to their N ‐acyl methyl ester analogs, and their identities were confirmed by GC/MS analysis. The sample recovery was quantitative (>98%), and the column matrix was very resilient, as demonstrated by consistent separation of the solutes after ∼100 preparative cycles.