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Preparative isolation of flavonoid compounds from Oroxylum indicum by high‐speed counter‐current chromatography by using ionic liquids as the modifier of two‐phase solvent system
Author(s) -
Liu Renmin,
Xu Lili,
Li Aifeng,
Sun Ailing
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900612
Subject(s) - chemistry , baicalein , chromatography , flavonoid , countercurrent chromatography , high performance liquid chromatography , solvent , glucoside , ethyl acetate , organic chemistry , medicine , genetics , alternative medicine , biology , antioxidant , pathology
A preparative high‐speed counter‐current chromatography method for isolation and purification of flavonoid compounds from Oroxylum indicum was successfully established by using ionic liquids as the modifier of the two‐phase solvent system. Two flavonoid compounds including baicalein‐7‐ O ‐diglucoside and baicalein‐7‐ O ‐glucoside were purified from the crude extract of O. indicum by using ethyl acetate–water–[C 4 mim][PF 6 ] (5:5:0.2, v/v) as two‐phase solvent system. 36.4 mg of baicalein‐7‐ O ‐diglucoside and 60.5 mg of baicalein‐7‐ O ‐glucoside were obtained from 120 mg of the crude extract. Their purities were 98.7 and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by 1 H‐NMR and 13 C‐NMR.