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Simultaneous analysis of endogenetic and ectogenic plant hormones by pressurized capillary electrochromatography
Author(s) -
Lu Qiaomei,
Zhang Lan,
Chen Lihui,
Lu Minghua,
Tong Ping,
Chen Guonan
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900603
Subject(s) - capillary electrochromatography , chromatography , chemistry , electrochromatography , phosphate buffered saline , analytical chemistry (journal) , capillary action , linearity , volumetric flow rate , phase (matter) , high performance liquid chromatography , capillary electrophoresis , materials science , physics , organic chemistry , quantum mechanics , composite material
Pressurized CEC (pCEC) with UV detection was developed for the simultaneous analysis of endogenetic and ectogenic plant hormones. Effects of some crucial factors including mobile phase, buffer concentration, pH value and applied voltage on pCEC separation were investigated, respectively. Under the optimum conditions, namely a packed capillary column (3 μm Hypersil C 18 , 45 cm total length with 100 μm id×20 cm effective length) as the separation channel; ACN: 10 mmol/L phosphate (50:50, v/v, %) as the mobile phase, pH 4.0; pump flow rate 0.035 mL/min; separation voltage −10 kV; UV wavelength 222 nm, a complete separation of five hormones was accomplished. The pCEC assay presented good linearity (0.8–100 μg/mL), with LOD of 0.2 μg/mL and R in range of 0.998–0.999. Meanwhile, a lack‐of‐fit test was performed to evaluate these linear models. Results showed that no significant lack‐of‐fit was observed and these models were appropriate (α=0.05).