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Suitability of ionic liquids as mobile‐phase additives in HPLC with fluorescence and UV detection for the determination of heterocyclic aromatic amines
Author(s) -
MartínCalero Aurora,
Tejral Gracian,
Ayala Juan H.,
González Venerando,
Afonso Ana M.
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900596
Subject(s) - tetrafluoroborate , ionic liquid , chemistry , triethylamine , silanol , fluorescence , high performance liquid chromatography , chromatography , phase (matter) , fluorescence spectroscopy , organic chemistry , catalysis , physics , quantum mechanics
Abstract The effects of several ionic liquids (ILs) as mobile‐phase additives in HPLC with fluorescence and UV–Vis detection for the determination of six heterocyclic aromatic amines were evaluated using two different C 18 stationary phases with moderate silanol activity. The studied ILs were 1‐butyl‐3‐methylimidazolium tetrafluoroborate, 1‐hexyl‐3‐methylimidazolium tetrafluoroborate and 1‐methyl‐3‐octylimidazolium tetrafluoroborate. The optical behaviour of heterocyclic aromatic amines in presence of ILs was studied and the silanol‐suppressing potency of ILs was evaluated for the two stationary phases studied. Several chromatographic parameters were evaluated in the presence or absence of ILs, or using triethylamine, the most common mobile‐phase additive. The best results were achieved using 1 mM 1‐butyl‐3‐methylimidazolium tetrafluoroborate as mobile‐phase additive and NovaPak ® column. In these conditions and with 18% of ACN in the mobile phase, analytical performance of the chromatographic methods using fluorescence and UV–Vis were evaluated, obtaining good precision in all cases (RSD lower than 6.6%) and low LOD (0.001–0.147 μg/mL with UV–Vis and 0.001–0.006 ng/mL with fluorescence detection).