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Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection
Author(s) -
Wang Shuang,
Yang Fengqing,
Feng Kun,
Li Deqiang,
Zhao Jing,
Li Shaoping
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900570
Subject(s) - cordyceps , chromatography detector , chromatography , chemistry , repeatability , high performance liquid chromatography , nucleobase , detection limit , analytical chemistry (journal) , biochemistry , dna , food science
A HPLC coupled with diode array detection (DAD) and evaporative light scattering detection (ELSD) method for qualitative and quantitative analysis of eight nucleosides and nucleobases, three carbohydrates and myriocin in Cordyceps was developed . A Prevail Carbohydrate ES column was employed for the separation within 50 min. Nucleosides and their bases were tested at UV 254 nm. ELSD was connected with DAD to determine myriocin and carbohydrates. The optimum drift tube temperature of ELSD was at 94°C with the nitrogen flow rate of 2.0 L/min. All calibration curves showed good linearity ( R 2 >0.9933) during the test ranges. The precision, repeatability, accuracy, LOD and LOQ were also fully investigated. This developed method was successfully applied to quantify 12 components, eight nucleosides and nucleobases, three carbohydrates and myriocin, in natural and cultured Cordyceps , which provides another view for quality control of Cordyceps sinensis .