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Optimization of supercritical fluid extraction of saikosaponins from Bupleurum falcatum with orthogonal array design
Author(s) -
Sun Yinshi,
Wei Li,
Wang Jianhua,
Bi Jianjie,
Liu Zhengbo,
Wang Ying,
Guo Zude
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900529
Subject(s) - chromatography , chemistry , supercritical fluid extraction , extraction (chemistry) , bupleurum , supercritical fluid , ethanol , high performance liquid chromatography , organic chemistry , medicine , alternative medicine , traditional chinese medicine , pathology
Supercritical fluid extraction (SFE) was used to extract saikosaponins a, c and d from the root of Bupleurum falcatum . An orthogonal array design L 9 (3) 4 was employed as a chemometric method for the optimization of the SFE conditions. The effects of four factors including pressure (30–40 MPa), temperature (40–50°C), ethanol concentration (60–100%) and time (2.5–3.5 h) on the yields of saikosaponins were investigated by a preparative SFE system in the SFE mode. Under the optimized conditions, namely 35 MPa of pressure, 45°C of temperature, 80% of ethanol concentration and 3.0 h of time, the yields of saikosaponin c, saikosaponin a, saikosaponin d, total saikosaponins and SFE extract were 0.16, 0.12, 0.96, 1.24 and 16.48 mg/g, respectively. Determinations of the saikosaponins were performed by HPLC.