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Preparative isolation of neomangiferin and mangiferin from Rhizoma anemarrhenae by high‐speed countercurrent chromatography using ionic liquids as a two‐phase solvent system modifier
Author(s) -
Xu Lili,
Li Aifeng,
Sun Ailing,
Liu Renmin
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900528
Subject(s) - mangiferin , chromatography , countercurrent chromatography , chemistry , solvent , high performance liquid chromatography , organic chemistry
A preparative high‐speed countercurrent chromatography method for isolation and purification of neomangiferin and mangiferin from Rhizoma anemarrhenae was successfully established by using ionic liquids as the modifier of the two‐phase solvent system. Neomangiferin and mangiferin were purified from the crude extract of R. anemarrhenae by using ethyl acetate‐water‐[C 4 mim][PF 6 ] (5:5:0.2 v/v) as two‐phase solvent system. In total, 22.5 mg of neomangiferin and 70.6 mg of mangiferin were obtained from 150 mg of the crude extract. The purities of neomangiferin and mangiferin were 97.2 and 98.1%, respectively, as determined by HPLC. The chemical structures of the isolated compounds were identified by 1 H‐NMR and 13 C‐NMR.