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Determination of volatile organic acids in tobacco by single‐drop microextraction with in‐syringe derivatization followed by GC‐MS
Author(s) -
Sha Yunfei,
Meng Jiaoran,
Zhang Yichun,
Deng Chunhui,
Wu Da
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900523
Subject(s) - derivatization , chromatography , chemistry , reagent , syringe , extraction (chemistry) , sample preparation , solid phase microextraction , gas chromatography , volatile organic compound , gas chromatography–mass spectrometry , high performance liquid chromatography , mass spectrometry , organic chemistry , psychology , psychiatry
In this work, the novel technique based on headspace single‐drop microextraction with in‐syringe derivatization followed by GC‐MS was established to determine the volatile organic acids in tobacco. The parameters for headspace single‐drop microextraction and in‐syringe derivatization were optimized, including extraction time, and volume of derivatization reagent and in‐syringe derivatization time. The method validations including linearity, precision, recovery and LOD were also studied. The obtained results illustrated that the optimized technique was easy, highly efficient and sensitive. Finally, the proposed method was successfully applied to the analyses of volatile organic acids in tobacco samples with seven different brands. It was further demonstrated that the present technique developed in this study does offer a simple and fast approach to determine volatile organic acids in tobacco.