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Rapid analysis of alkylphosphonate drugs by capillary zone electrophoresis using indirect ultraviolet detection
Author(s) -
Prutthiwanasan Brompoj,
Suntornsuk Leena
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900518
Subject(s) - chemistry , capillary electrophoresis , chromatography , analyte , resolution (logic) , detection limit , analytical chemistry (journal) , electrophoresis , computer science , artificial intelligence
A rapid capillary electrophoretic method for the analysis of three alkylphosphonate drugs ( i.e. fosfomycin disodium (FOS), clodronate disodium (CLO) and alendronate sodium (ALN)) was developed by using multiple probe BGE and indirect UV detection. BGE containing 30 mM benzoic acid, 5 mM salicylic acid and 0.5 mM CTAB (pH 3.8), temperature of 30°C, applied voltage of −30 kV and detection at 220 nm provided baseline separation of all analytes (resolution ( R )>2.2) in 3.2 min. EOF reversal by addition of CTAB and negative voltage polarity leading to the co‐EOF flow and short analysis time. Two probe BGE greatly improved peak symmetry. The method showed good linearity ( r 2 >0.999 in ranges of 20–1000 μg/mL for FOS, 100–1000 μg/mL for CLO and 100–750 μg/mL for ALN) repeatablitiy (RSD<2.15%), recovery (99.3–101.1%) and sensitivity (LOD<50 μg/mL). Freshly prepared BGE and sample solutions are essential for the method precision and accuracy. This new method can be utilized for routine analysis of FOS, CLO and ALN in dosage forms because of its efficiency, reliability, speed and simplicity.