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Investigation of relationships between barley stress peptides and beer gushing using SDS‐PAGE and MS screening
Author(s) -
Hégrová Blanka,
Farková Marta,
Macuchová Simona,
Havel Josef,
Preisler Jan
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900510
Subject(s) - proteome , plant lipid transfer proteins , antifungal , chromatography , chemistry , peptide , gel electrophoresis , food science , biochemistry , biology , microbiology and biotechnology , gene
The relationship between gushing and antifungal peptides in barley and malt kernels was examined for five barley varieties produced in the Czech Republic with four conditions of infection and treatment. Proteome changes during pathogen‐seed interaction were observed with SDS‐PAGE and MALDI‐TOF MS. These methods were applied as a fast screening for observing the relationship between gushing and peptides/proteins. It was found that the presence of basic peptides, presumably hordothionins and non‐specific lipid transfer protein type 1, did not correlate with the degree of gushing for malt (∣ r ∣∈<0.07, 0.34>), (∣ r ∣∈<0.01, 0.49>), respectively, as detected by both methods.