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Screening of closantel and rafoxanide in animal muscles by HPLC with fluorescence detection and confirmation using MS
Author(s) -
Yeung Hoisze,
Lee Waion,
Wong Yiutung
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900503
Subject(s) - chromatography , residue (chemistry) , high performance liquid chromatography , chemistry , maximum residue limit , biology , pesticide residue , biochemistry , pesticide , agronomy
A sensitive and selective LC‐FLD method was described for determination of closantel and rafoxanide in bovine and ovine muscles. Bovine and ovine muscles were extracted with ACN and acetone mixture (80:20, v/v). After cleanup with Oasis ® MAX SPE cartridges, the sample was analyzed by LC‐FLD using the control point approach. No false‐negative result was observed at or below maximum residue limits and the false‐positive rate was below 5%. Suspected positive sample was confirmed by LC‐MS/MS. This method was suitable for screening of large batch of samples and hence considerably reduced the time and cost required for quantitation and confirmatory analyses.