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Capillary chromatography based on tube radial distribution of aqueous‐organic mixture carrier solvents: Introduction of inner‐wall‐modified capillary tubes
Author(s) -
Jinno Naoya,
Tsuji Katsuya,
Shikatani Kaoru,
Hashimoto Masahiko,
Tsukagoshi Kazuhiko
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900470
Subject(s) - chemistry , iminodiacetic acid , capillary action , chromatography , aqueous solution , solvent , phenylboronic acid , ethyl acetate , buffer solution , capillary electrophoresis , inorganic chemistry , organic chemistry , materials science , composite material , catalysis , chelation
We developed a novel capillary chromatography using an open capillary tube and a water‐hydrophilic/hydrophobic organic solvent mixture as a carrier solution. The capillary chromatography was called a tube radial distribution chromatography (TRDC) system. In this study we tried to introduce inner‐wall‐modified ( e.g. phenylboronic‐acid‐ and iminodiacetic‐acid‐modified) fused‐silica capillary tubes to the TRDC system to separate model mixture analytes. The phenylboronic‐acid‐modified capillary tube was combined with absorption detection to analyze a mixture of adenosine and deoxyadenosine. The iminodiacetic‐acid‐modified capillary tube was combined with chemiluminescence detection using a luminol reaction to analyze a mixture of copper(II) and hematin. A water (carbonate buffer)/ACN/ethyl acetate (2:7:4 v/v/v) and a water (carbonate buffer)/ACN/ethyl acetate (15:3:2 v/v/v) mixture solution were used as carrier solutions in the TRDC system, and typical carbonate buffer solutions not containing any organic solvents were also used as carrier solutions as reference solutions. In both modified capillary tubes, the organic‐solvent‐rich carrier solution successfully improved the separation of the mixture analytes in the system, and the water‐rich carrier solution greatly depressed their separation, when compared with chromatography using carbonate buffer carrier solutions containing no organic solvents. Such observed phenomena were discussed considering the separation mechanism of the TRDC system.