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A GC – magnetic sector MS method for identification and quantification of fatty acids in ewe milk by different acquisition modes
Author(s) -
Devle Hanne,
Rukke EllingOlav,
NaessAndresen Carl Fredrik,
Ekeberg Dag
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900455
Subject(s) - chromatography , chemistry , gas chromatography , fatty acid , quantitative analysis (chemistry) , calibration , gas chromatography–mass spectrometry , electron capture detector , analytical chemistry (journal) , mass spectrometry , mathematics , organic chemistry , statistics
A general method for qualitative and quantitative determination of fatty acids (FAs) using GC‐MS was developed and tested on ewe milk. A total number of 38 poly unsaturated FAs, monounsaturated FAs and saturated FAs, from C6:0 to C24:1, were used in a comparative study of scan, reconstructed ion chromatogram and SIM. Fatty acid methyl esters in standard solutions as well as in milk from ewe were analyzed by these techniques, using a sector instrument. Instrument precision, linearity, LOD and LOQ, as well as calibration behavior and response factors were investigated for each approach. The quantitative results obtained by each technique were compared. All techniques had values for LOD and LOQ in the ng/mL region.