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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high‐performance centrifugal partition chromatography
Author(s) -
Dang YuanYe,
Li XiaoCen,
Zhang QingWen,
Li ShaoPing,
Wang YiTao
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900453
Subject(s) - chemistry , chromatography , acetone , essential oil , petroleum ether , curcuma , extraction (chemistry) , organic chemistry , traditional medicine , medicine
Six volatile compounds, curdione ( 1 ), curcumol ( 2 ), germacrone ( 3 ), curzerene ( 4 ), 1,8‐cineole ( 5 ) and β‐elemene ( 6 ), were successfully isolated from the essential oil of Curcuma wenyujin by high‐performance centrifugal partition chromatography using a nonaqueous two‐phase solvent system consisting of petroleum ether‐acetonitrile‐acetone (4:3:1 v/v/v). A total of 8 mg of curdione ( 1 ), 4 mg of curcumol ( 2 ), 10 mg of germacrone ( 3 ), 18 mg of curzerene ( 4 ), 9 mg of 1,8‐cineole ( 5 ) and 17 mg of β‐elemene ( 6 ) were isolated from the essential oil (300 mg) in 500 min. Their structures were determined by comparison of their retention times and MS data with those of the authentic samples as well as NMR spectroscopic analysis.