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Lippia origanoides chemotype differentiation based on essential oil GC‐MS and principal component analysis
Author(s) -
Stashenko Elena E.,
Martínez Jairo R.,
Ruíz Carlos A.,
Arias Ginna,
Durán Camilo,
Salgar William,
Cala Mónica
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900452
Subject(s) - chemotype , carvacrol , essential oil , thymol , limonene , chemistry , carvone , eugenol , lippia , chromatography , food science , botany , biology , organic chemistry
Chromatographic (GC/flame ionization detection, GC/MS) and statistical analyses were applied to the study of essential oils and extracts obtained from flowers, leaves, and stems of Lippia origanoides plants, growing wild in different Colombian regions. Retention indices, mass spectra, and standard substances were used in the identification of 139 substances detected in these essential oils and extracts. Principal component analysis allowed L. origanoides classification into three chemotypes, characterized according to their essential oil major components. α‐ and β‐phellandrenes, p ‐cymene, and limonene distinguished chemotype A; carvacrol and thymol were the distinctive major components of chemotypes B and C, respectively. Pinocembrin (5,7‐dihydroxyflavanone) was found in L. origanoides chemotype A supercritical fluid (CO 2 ) extract at a concentration of 0.83±0.03 mg/g of dry plant material, which makes this plant an interesting source of an important bioactive flavanone with diverse potential applications in cosmetic, food, and pharmaceutical products.