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Reversed phase capillary HPLC using polymer‐entrapped C18 particles
Author(s) -
Gibson Graham T. T.,
Marecak Dale M.,
Oleschuk Richard D.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900447
Subject(s) - high performance liquid chromatography , chromatography , capillary action , polymer , chemistry , reversed phase chromatography , phase (matter) , capillary electrophoresis , materials science , organic chemistry , composite material
Novel capillary columns containing polymer‐entrapped octadecylsilyl‐modified silica microspheres were evaluated for chromatographic performance. The polymer forms only at the particle surface and in as little as 10 s strongly immobilizes them, obviating the need for a frit. The photoinitiated polymerization is patternable, and various entrapment styles were compared, all of which withstood pressure drops of at least 5000 psi (345 bar). The fritless nature of the columns allows a unique mass production capability whereby long packed columns are entrapped and simply cut afterward. Since the material has previously been shown to be a proficient nanoelectrospray emitter, the columns have enormous potential for use in capillary LC‐MS. The evaluation suggests that for the designs that have minimal entrapped regions, the column performance is equivalent to commercial columns containing similar particles.