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Simultaneous determination of five tetracycline and macrolide antibiotics in feeds using HPCE
Author(s) -
Tong Jing,
Rao Qinxiong,
Zhu Kui,
Jiang Zhigang,
Ding Shuangyang
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900415
Subject(s) - tilmicosin , tylosin , oxytetracycline , tetracycline , chromatography , doxycycline , chemistry , repeatability , tetracycline antibiotics , antibiotics , capillary electrophoresis , analyte , detection limit , chlortetracycline , spiramycin , citric acid , macrolide antibiotics , erythromycin , biochemistry
This work demonstrates the potential of HPCE in the analysis of antibiotics in a complex matrix such as feedstuffs. Using 20 mM citric acid–40 mM Na 2 HPO 4 buffer (pH 2.65), the five antibiotics, tetracycline, oxytetracycline, doxycycline, tilmicosin, and tylosin were successfully separated at 30 kV in a 64.5 cm×75 μm id capillary. Good repeatability, stability, and reliability of the method were supported by <10% CV with mean recoveries of >70%, and the limit of detection of the five analytes was 0.5–1 mg/kg. It was for the first time that a capillary electrophoretic method was employed to simultaneously detect five tetracycline and macrolide antibiotics in animal feeds.