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Simultaneous determination of avermectins in bovine tissues by LC‐MS/MS
Author(s) -
Inoue Koichi,
Yoshimi Yukiko,
Hino Tomoaki,
Oka Hisao
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900411
Subject(s) - chromatography , chemistry , repeatability , ammonium formate , formic acid , reproducibility , electrospray , analyte , extraction (chemistry) , detection limit , calibration curve , benzamidine , sample preparation , ammonium acetate , high performance liquid chromatography , formate , biochemistry , enzyme , catalysis
Analytical method for the simultaneous quantification of avermectins (AVMs), abamectin B1a, abamectin 8,9‐Z isomer B1a, emamectine benzoate B1a, emamectine benzoate 8,9‐Z isomer B1a, ivermectin, eprinomectin B1a, doramectin and moxidectin in bovine tissues (muscle, liver and fat) was developed by LC‐MS/MS in electrospray positive ion mode. The separation was achieved on a short TSK‐GEL ODS 100V column with the mobile phase consisting of acetonitrile and aquatic 0.1 mM ammonium formate containing 0.1% formic acid v/v at a flow rate of 0.2 mL/min with gradient elution. Liquid–liquid extraction with isooctane was used for the sample extraction/preparation of analytes in bovine samples. The linearity of the calibration curves was excellent in matrix‐matched standards, and yielded the coefficients ( r 2 =0.997–0.999, range from LOQ to 500, 1000 or 5000 ng/g) of determination of the target analytes. Recoveries were in the range of 87.9–99.8% with associated precision values (within‐day: 1.5–7.4%, n =6, and between‐day: 1.5–8.4% for 3 days) for repeatability and reproducibility. LC‐MS/MS method has been proven to be highly efficient and suitable for the simultaneous determinations of eight AVMs in bovine tissue samples.