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Isolation of inulin‐type oligosaccharides from Chinese traditional medicine: Morinda officinalis How and their characterization using ESI‐MS/MS
Author(s) -
Yang Zhenmin,
Yi Yongtao,
Gao Chuanchuan,
Hou Dengke,
Hu Jun,
Zhao Mingyue
Publication year - 2010
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900396
Subject(s) - glycosidic bond , chemistry , inulin , oligosaccharide , chromatography , mass spectrometry , size exclusion chromatography , organic chemistry , biochemistry , enzyme
Inulin‐type oligosaccharides with different DP were prepared by size‐exclusion chromatography and purity of each oligosaccharide was determined by HPLC equipped with cyclodextrin‐bond column. The purities of obtained inulin‐type oligosaccharides with different DP were more than 98% by one‐step process. The DP and molecular weight were obtained through ESI‐MS in negative mode. The characterization of the inulin‐type oligosaccharides with different DP was studied by MS/MS spectra obtained by collision‐induced dissociation of molecular ions ([M−H] − ). When the DP was lower, the fragment ions were formed through cross‐ring cleavages of two bonds within the sugar ring and glycosidic cleavages. However, with the increase of DP, the ions resulting from glycosidic cleavages between two sugar residues were predominant.