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Determination of methylene blue residues in aquatic products by liquid chromatography‐tandem mass spectrometry
Author(s) -
Xu JinZhong,
Dai Lin,
Wu Bin,
Ding Tao,
Zhu JunJie,
Lin Hong,
Chen HuiLan,
Shen ChongYu,
Jiang Yuan
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900364
Subject(s) - chemistry , chromatography , extraction (chemistry) , methylene blue , detection limit , solid phase extraction , mass spectrometry , dichloromethane , cartridge , liquid chromatography–mass spectrometry , acetonitrile , dissolution , liquid–liquid extraction , tandem mass spectrometry , solvent , mechanical engineering , biochemistry , organic chemistry , photocatalysis , engineering , catalysis
A method for the determination and confirmation of methylene blue (MB) in aquatic products was developed. Residues of MB were extracted from homogenized tissues with acetonitrile/sodium acetate buffer solution, and simply cleaned up with dichloromethane liquid/liquid extraction. After concentration and dissolution, the sample solutions were cleaned up by the neutral alumina and weak cation‐exchange solid phase extraction (SPE) cartridge, prior to LC‐MS/MS analysis. MB was determined at 1.0–20 μg/kg in eel, toasted eel and shrimp, with a limit of quantification of 0.5 μg/kg. Recovery for MB was between 73.0% and 108.3%. This method is fast, exact and sensitive. It can be applied to determine MB in aquatic products.