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Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules
Author(s) -
Roberts Michael W. H.,
Ongkudon Clarence M.,
Forde Gareth M.,
Danquah Michael K.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900309
Subject(s) - biomolecule , glycidyl methacrylate , monolith , adsorption , monolithic hplc column , chemistry , chromatography , chemical engineering , methacrylate , membrane , copolymer , materials science , polymer , nanotechnology , organic chemistry , high performance liquid chromatography , biochemistry , engineering , catalysis
Polymethacrylate monoliths, specifically poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate) or poly(GMA‐ co‐ EDMA) monoliths, are a new generation of chromatographic supports and are significantly different from conventional particle‐based adsorbents, membranes, and other monolithic supports for biomolecule purification. Similar to other monoliths, polymethacrylate monoliths possess large pores which allow convective flow of mobile phase and result in high flow rates at reduced pressure drop, unlike particulate supports. The simplicity of the adsorbent synthesis, pH resistance, and the ease and flexibility of tailoring their pore size to that of the target biomolecule are the key properties which differentiate polymethacrylate monoliths from other monoliths. Polymethacrylate monoliths are endowed with reactive epoxy groups for easy functionalization (with anion‐exchange, hydrophobic, and affinity ligands) and high ligand retention. In this review, the structure and performance of polymethacrylate monoliths for chromatographic purification of biomolecules are evaluated and compared to those of other supports. The development and use of polymethacrylate monoliths for research applications have grown rapidly in recent times and have enabled the achievement of high through‐put biomolecule purification on semi‐preparative and preparative scales.