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Direct characterization of aqueous extract of Hibiscus sabdariffa using HPLC with diode array detection coupled to ESI and ion trap MS
Author(s) -
RodríguezMedina Inmaculada C.,
BeltránDebón Raúl,
Molina Vicente Micol,
AlonsoVillaverde Carlos,
Joven Jorge,
Menéndez Javier A.,
SeguraCarretero Antonio,
FernándezGutiérrez Alberto
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900298
Subject(s) - chemistry , chromatography , electrospray ionization , high performance liquid chromatography , hibiscus sabdariffa , mass spectrometry , tandem mass spectrometry , ion trap , aqueous solution , electrospray , analytical chemistry (journal) , organic chemistry , food science
The phenolic fraction and other polar compounds of the Hibiscus sabdariffa were separated and identified by HPLC with diode array detection coupled to electrospray TOF and IT tandem MS (DAD‐HPLC‐ESI‐TOF‐MS and IT‐MS). The H. sabdariffa aqueous extract was filtered and directly injected into the LC system. The analysis of the compounds was carried out by RP HPLC coupled to DAD and TOF‐MS in order to obtain molecular formula and exact mass. Posterior analyses with IT‐MS were performed and the fragmentation pattern and confirmation of the structures were achieved. The H. sabdariffa samples were successfully analyzed in positive and negative ionization modes with two optimized linear gradients. In positive mode, the two most representative anthocyanins and other compounds were identified whereas the phenolic fraction, hydroxycitric acid and its lactone were identified using the negative ionization mode.