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Capillary electrophoresis with dual laser detection in separation of amplified fragment length polymorphism fragments
Author(s) -
StefanowiczHajduk Justyna,
Filipowicz Natalia,
Kosinski Igor,
Ochocka J. Renata
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900297
Subject(s) - amplified fragment length polymorphism , capillary electrophoresis , chromatography , chemistry , dna sequencer , dna , fluorescence , fragment (logic) , analytical chemistry (journal) , microbiology and biotechnology , biology , dna sequencing , biochemistry , population , physics , demography , sociology , computer science , genetic diversity , programming language , quantum mechanics
We are presenting the application of CE technique with dual‐channel LIF detection for the simultaneous separation of DNA fragments labeled with two different fluorescence dyes. The optimal conditions of the analysis were determined for the separation of amplified fragment length polymorphism (AFLP) fragments labeled with 5′‐6‐carboxyfluorescein (6‐FAM) and the DNA size standard labeled with sulfoindocyanine succinimidyl ester (Cy‐5). CE equipped with both argon ion and diode lasers is a good alternative for sequencers and might be applied in analyses of PCR products generated by various fingerprinting methods.