Premium
Performance of a Chromolith RP‐18e column for the screening of β‐blockers
Author(s) -
PousTorres Sandra,
RuizÁngel MariaJosé,
TorresLapasió José Ramón,
GarcíaÁlvarezCoque Maria Celia
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900291
Subject(s) - chromatography , chemistry , high performance liquid chromatography , column (typography) , analytical chemistry (journal) , computer science , telecommunications , frame (networking)
The chromatographic performance of a monolithic column (Chromolith RP‐18e) was comprehensively examined in the isocratic separation of ten β‐blockers, using ACN–water mobile phases, and compared with the performance of three microparticulate RP columns manufactured with different types of silica: Spherisorb ODS‐2, Kromasil C18 and XTerra MS C18. The comparison considered the analysis time, selectivity, peak shape (column efficiency and asymmetry) and resolution, and was extended to a wide range of mobile phase compositions. The Chromolith column showed good performance for the analysis of β‐blockers with regard to the packed columns. In terms of selectivity and analysis time, the greatest similarity was found between the Chromolith and XTerra columns. The addition of a silanol blocking agent (0.1% triethylamine) to both Chromolith and Spherisorb columns yielded, apparently, a similar blocking degree of the silanol groups (based on the similar peak shapes), and gave rise to similar selectivity.