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Modified TLC bioautographic method for screening acetylcholinesterase inhibitors from plant extracts
Author(s) -
Yang Zhongduo,
Zhang Xu,
Duan Dongzhu,
Song Zhuwen,
Yang Mingjun,
Li Shuo
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900266
Subject(s) - chemistry , acetylcholinesterase , chromatography , reagent , enzyme , spots , enzyme assay , salt (chemistry) , biochemistry , organic chemistry
Abstract The present work describes modifications to an existing TLC bioautographic method for detecting acetylcholinesterase inhibitors from plant extracts. The basic principle of the method is that the enzyme converts 1‐naphthyl acetate into naphthol which reacts with Fast Blue B salt to make a purple‐colored background on the TLC plates. Inhibitors of acetylcholinesterases produced white spots on the background. Our modifications involve changes in the concentration of the enzyme, the reagents, and the time of the reaction. With these changes, the consumption of the enzyme was reduced by 85% and the detection limits were decreased remarkably.