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Comparison between monolithic and particle‐packed platinum C 18 columns in HPLC determination of acidic and basic test mixtures
Author(s) -
Atia Noha N.,
York Peter,
Clark Brian J.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900254
Subject(s) - van deemter equation , chromatography , chemistry , theoretical plate , analytical chemistry (journal) , reproducibility , platinum , column (typography) , resolution (logic) , particle size , high performance liquid chromatography , monolithic hplc column , volumetric flow rate , particle (ecology) , mathematics , geometry , biochemistry , physics , oceanography , connection (principal bundle) , quantum mechanics , artificial intelligence , computer science , geology , catalysis
Herein we report the results of a comparative study on the performance of Monolithic RP‐18e and Platinum C 18 3 μm columns for isocratic separation of acidic and basic test compounds. The inter‐ and intraday precision of different practical parameters including number of theoretical plates ( N ), capacity factor ( K ′), tailing factor ( T 0.05 ), and resolution ( R s ) were determined for both columns. Two different production batches were used for each column and batch to batch reproducibility of both columns was evaluated. The column backpressure drop over flow rate range 0.5–2 mL/min at the monolithic columns was two‐ to three‐times lower than that on the platinum column without loss of the column efficiency. The plate heights were used to estimate the columns efficiency using Van Deemter plots. Both types of columns were able to separate the tested compounds well with sufficient resolution and peak symmetry but they differ in the analysis time and column backpressure, significantly. Monolithic column was more convenient as it enables the analytical run under low backpressure at shorter time with sufficient separation efficiency.