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Molecular imprinting of AMP by an ionic‐noncovalent dual approach
Author(s) -
Breton Florent,
Delépée Raphaël,
Agrofoglio Luigi A.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900226
Subject(s) - moiety , methacrylate , chemistry , molecularly imprinted polymer , monomer , nucleotide , molecular imprinting , acrylamide , adenylate kinase , polymer , adenosine monophosphate , combinatorial chemistry , adenosine , imprinting (psychology) , enzyme , stereochemistry , organic chemistry , biochemistry , selectivity , catalysis , gene
In order to mimic recognition properties of adenylate kinase, molecularly imprinted polymers (MIPs) were prepared for adenosine 5′‐monophosphate (AMP), a substrate of the enzyme. Different functional monomers interacting with the phosphate moiety were tested, and the MIP giving the best specific binding of AMP was composed with one equivalent of 2‐(dimethylamino)ethyl methacrylate and ten equivalents of acrylamide compared to AMP. Packed into solid phase cartridge, this polymer showed similar characteristics than the enzyme, since it was specific for AMP toward other nucleotides.