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Microchip free flow planar reversed phase electrochromatography with monolithic stationary phase
Author(s) -
Wang Pingli,
Tao Dingyin,
Zhang Lihua,
Liang Zhen,
Zhang Yukui
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900223
Subject(s) - electrochromatography , polymerization , microscale chemistry , capillary electrochromatography , materials science , monolith , monolithic hplc column , chromatography , phase (matter) , chemistry , analytical chemistry (journal) , chemical engineering , polymer , high performance liquid chromatography , capillary electrophoresis , organic chemistry , composite material , mathematics education , mathematics , catalysis , engineering
In this study, microchip free flow planar RP electrochromatography (μFF‐PRPEC) was developed by in situ polymerization of monolithic materials in microchamber, and successfully applied for the separation of dyes and proteins. Poly(butyle methyacrylate‐ co ‐ethylene dimethacrylate) was prepared by UV‐initiated polymerization in a glass microchamber (42 mm long, 23 mm wide, and 28 μm deep). A mixture of 1‐propanol, 1,4‐butanediol, and water was chosen as porogens, and 1.2% (wt%) 2‐acrylamide‐2‐methyl‐propanesulfonic acid (AMPS) was added into the polymerization solution to generate EOF. With 30% v/v ACN‐15 mM Tris‐HCl as the mobile phase, rhodamine B and methyl green were separated from each other with 400 V transverse voltage applied, and resolution as high as 4.6 was obtained, much higher than that obtained by μFFE under optimal conditions. Furthermore, μFF‐PRPEC was also successfully applied into the separation of lysozyme and ribonuclease B, and resolution as high as 9.4 was obtained. All these results demonstrate that μFF‐RPPEC might have great potential in the microscale continuous preparation of samples with improved resolution compared to μFFE.