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Identification of volatile compounds of Atractylode lancea Rhizoma using supercritical fluid extraction and GC–MS
Author(s) -
Chen Qinhua,
Li Peng,
Yang Handong,
Li Xiulou,
Zhu Jun,
Chen Fuchao
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900210
Subject(s) - chromatography , supercritical fluid extraction , supercritical fluid , chemistry , extraction (chemistry) , gas chromatography–mass spectrometry , mass spectrometry , supercritical fluid chromatography , gas chromatography , organic chemistry
Supercritical fluid was used to extract volatile components from the rhizoma of Atractylode lancea ( A. lancea ). An orthogonal array design (OAD), L 9 (3) 4 , was employed as a chemometric method for the optimization of the supercritical fluid extraction (SFE) of volatile compounds from the herbal medicine. Four parameters, namely, pressure, temperature, dynamic extraction time, and flow rate of CO 2 , were studied and optimized by a three‐level OAD in which the interactions between the parameters were neglected. These compounds were identified according to their retention times and mass spectra by GC–MS. A total of 30 compounds of SFE extracts were identified. Atractylon (8.63%), hinesol (1.44%), β‐eudesmol (6.64%), elemol (0.42%), and atractydin (13.92%) were the major sesquiterpenes identified in A. lancea SFE extracts.