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Selective molecularly imprinted stationary phases for Bisphenol A analysis prepared by modified precipitation polymerization
Author(s) -
Jiang Ming,
Shi Yun,
Zhang Renli,
Shi Changhua,
Peng Yan,
Huang Zheng,
Lu Bin
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900207
Subject(s) - molecularly imprinted polymer , precipitation polymerization , chromatography , dispersity , bisphenol a , monomer , chemistry , polymerization , repeatability , suspension polymerization , bisphenol , molecular imprinting , detection limit , polymer , radical polymerization , polymer chemistry , selectivity , organic chemistry , catalysis , epoxy
Bisphenol A (BPA)‐imprinted polymeric microspheres were synthesized by modified precipitation polymerization (MPP) method. Influences of cross‐linker, monomer, porogen volume, and agitation on polymerization were investigated. Proper amount of cross‐linker ethyleneglycol‐dimethacrylate (EGDMA) was critical to achieve narrowly dispersed microspheres. For template BPA, monomer 4‐vinylpyridine (4‐VP) was better than MAA to get the best imprinted effects. The optimum template/monomer ratio was 1:6. Increasing porogen volume increased size dispersity and decreased binding characters. Agitation increased coagulation and resulted in irregular particles. Microspheres with the best binding characters were used as selective stationary phase of chromatographic column to detect BPA in milk, pig urine, and chicken meat. Under optimal chromatographic conditions, the calibration graph was linear with R 2 = 0.9994 in the range of 3–50 μmol/L. The LOD and LOQ were 1 and 3 μmol/L, respectively. When large amounts (20 mL or 20 g) of samples were analyzed, the recoveries ranged from 70.2 to 87.3% with RSD less than 4.85% in all samples spiked with 0.05–0.2 μmol/L BPA. The intra‐day and inter‐day RSD were less than 1.83 and 3.96%, respectively. Microspheres prepared by MPP are successfully used in molecularly imprinted polymer (MIP)‐based analytical column to detect trace BPA in different biologic samples with acceptable accuracy and repeatability.