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PEG enhances viral clearance on ceramic hydroxyapatite
Author(s) -
Snyder Mark A.,
Ng Paul,
Mekosh Heather,
Gag Pete
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900156
Subject(s) - peg ratio , elution , ethylene glycol , chemistry , ceramic , sodium , chromatography , buffer (optical fiber) , chloride , nuclear chemistry , organic chemistry , telecommunications , finance , computer science , economics
Viral clearance across ceramic hydroxyapatite (CHT™) was examined in two elution systems: sodium chloride and sodium chloride plus poly(ethylene glycol) (PEG). In both cases clearance of xenotropic murine leukemia virus was significant (3–4 log) while that of minute virus of mice varied between 1.7 and 2.7 log; in addition, the addition of PEG to the elution buffer enhanced viral clearance. The data are in agreement with the previous results and demonstrate that additional clearance can be obtained by adding PEG to a ceramic hydroxyapatite buffer system.