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Quantitation of the main constituents of vanilla by reverse phase HPLC and ultra‐high‐pressure‐liquid‐chromatography with UV detection: Method validation and performance comparison
Author(s) -
Cicchetti Esmeralda,
Chaintreau Alain
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900132
Subject(s) - chromatography , high performance liquid chromatography , chemistry , reversed phase chromatography
Vanilla's main constituents, i. e. , vanillin, para ‐hydroxybenzaldehyde, and their corresponding acids, can be easily quantified by RP LC with UV detection and external calibration. This paper describes two methods that were developed using HPLC and ultra‐high‐pressure LC (UHPLC), respectively, and validated according to the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) [1]. Both methods were highly specific, exhibited good linearities with high precision, and achieved good accuracies of quantitative results. The UHPLC method was more sensitive, five times shorter, and gave better peak resolutions than the HPLC alternative.