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The lipophilicity indices of flavonoids estimated by reversed‐phase liquid chromatography using different computation methods
Author(s) -
Briciu Rodica Domnica,
KotWasik Agata,
Namiesnik Jacek,
Sârbu Costel
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900106
Subject(s) - lipophilicity , chromatography , chemistry , reversed phase chromatography , high performance liquid chromatography , organic chemistry
The chromatographic behavior of some flavonoids was established by RP‐HPLC on RP‐18 (LiChroCART, LiChrosphere RP‐18e), RP‐8 (Zorbax, Eclipse XDB‐C8), CN (Säulentechnik, LiChrosphere CN100) columns. The mobile phases were mixtures of methanol‐water in different volume proportions from 70 to 80% v/v for RP‐18 and RP‐8, while for the CN column the proportions were between 66 and 70% v/v. The lipophilicity was expressed through different lipophilicity descriptors such as mean of k (mk), mean of log k (mlog k), log k W , S, f 0 , and scores of k and log k corresponding to the first principal component. The experimental lipophilicity indices are directly correlated with the computed values, via computer software and internet module, at a high analytical level. Furthermore, the results obtained applying principal component analysis to k and/or log k values allow the prediction and explanation of the interaction involved in the retention mechanism which takes place between the compounds and the employed stationary phases during the development.