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An immunoaffinity column with a monoclonal antibody as ligand for human follicle stimulating hormone
Author(s) -
Zandian Marlene,
Jungbauer Alois
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900103
Subject(s) - chromatography , chemistry , monoclonal antibody , chinese hamster ovary cell , affinity chromatography , follicle stimulating hormone , ligand (biochemistry) , luteinizing hormone , hormone , antibody , receptor , biochemistry , biology , immunology , enzyme
A detailed characterization regarding the chromatographic and biochemical properties of an immunoaffinity column for sample pretreatment using a follicle stimulating hormone (FSH) β‐unit specific mAb has been performed. A maximum binding capacity of approximately 300 μg/mL was determined. A K D value of 4.5×10 –8 M could be obtained in batch isotherm measurements. The dynamic binding capacity at 10% did not change when residence time was at least 6 min. High selectivity could be demonstrated using crude culture supernatant from FSH producing Chinese hamster ovary (CHO) cells. A great stability of the matrix was confirmed by performing 100 consecutive chromatographic runs without loss of capacity.