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Separation and sensitive detection of D ‐amino acids in biological matrices
Author(s) -
Kirschner Daniel L.,
Green Thomas K.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900101
Subject(s) - chemistry , chromatography , fluorescence , amino acid , high performance liquid chromatography , enantioselective synthesis , resolution (logic) , biochemistry , computer science , physics , quantum mechanics , artificial intelligence , catalysis
The increase in our understanding of D ‐amino acid function and distribution in mammals is in many ways a result of the initial development of sensitive enantioselective separation strategies that allow for quantification in real biological samples. This article reviews progress on the development of chiral selective separation and detection of D ‐amino acids including enzyme‐based microbiosensors, GC/MS, HPLC/fluorescence, HPLC/MS‐MS, cEKC/fluorescence, and MEKC/fluorescence. Only methods capable of analyzing D ‐isomers in biological matrices are given here and significant effort is made to highlight approaches that offer speed, resolution, high sensitivity, and versatility.