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Nucleic acid aptamer molecular recognition principles and application in liquid chromatography and capillary electrophoresis
Author(s) -
Peyrin Eric
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900061
Subject(s) - aptamer , capillary electrophoresis , nucleic acid , systematic evolution of ligands by exponential enrichment , molecular recognition , context (archaeology) , chemistry , chromatography , affinity electrophoresis , computational biology , rna , affinity chromatography , molecule , biochemistry , biology , microbiology and biotechnology , organic chemistry , paleontology , gene , enzyme
Nucleic acid aptamers isolated from the systematic evolution of ligands by exponential enrichment (SELEX) have the capacity to recognize various classes of target molecules with high affinity and specificity. In this context, the development of aptamer‐based molecular recognition tools has become a very interesting and promising analytical strategy during the last few years. In this review, the molecular recognition features of aptamers as well as the key factors for their practical applicability to the chromatographic and capillary electrophoretic fields are summarized.