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Rapid separation of three glucosylated resveratrol analogues from the invasive plant Polygonum cuspidatum by high‐speed countercurrent chromatography
Author(s) -
Fan Peihong,
Marston Andrew,
Hay AnneEmmanuelle,
Hostettmann Kurt
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900057
Subject(s) - chemistry , chromatography , countercurrent chromatography , piceatannol , high performance liquid chromatography , ethyl acetate , glucoside , methanol , column chromatography , reagent , resveratrol , organic chemistry , medicine , biochemistry , alternative medicine , pathology
Three glucosylated resveratrol analogues (piceid, piceatannol glucoside, resveratroloside) were successfully isolated from the crude MeOH extract of the invasive plant species Polygonum cuspidatum by semi‐preparative high‐speed countercurrent chromatography with a two‐phase solvent system composed of cyclohexane‐ethyl acetate‐methanol‐water (1:5:1:5, v/v/v/v). Piceid (23 mg), resveratroloside (17 mg), piceatannol glucoside (15 mg) of purities over 80% were isolated from 500 mg crude MeOH extract in one step. Subsequent passage over a SPE column was used to quickly bring their purities to over 90%. The purities were determined by HPLC analysis and their structures were elucidated by proton nuclear magnetic resonance ( 1 H‐NMR), HMBC, ESI‐MS and HR‐MS.