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Quantification of D ‐Asp and D ‐Glu in rat brain and human cerebrospinal fluid by microchip electrophoresis
Author(s) -
Huang Yong,
Shi Ming,
Zhao Shulin
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900026
Subject(s) - derivatization , chemistry , chromatography , cerebrospinal fluid , electrophoresis , gel electrophoresis , glutamate receptor , high performance liquid chromatography , biochemistry , biology , receptor , neuroscience
A microchip electrophoresis (MCE) method with LIF detection was presented for quantification of D ‐aspartic acid ( D ‐Asp) and D ‐glutamate ( D ‐Glu) in biological samples. D ‐Asp and D ‐Glu were determined after precolumn derivatization with FITC. The chiral separation was performed on a glass/PDMS hybrid microfluidic chip using γ‐CD as chiral selector in the running buffer. High sensitive detection was obtained by the LIF detection. The LODs (S/N = 3) for D ‐Asp and D ‐Glu were 6.0×10 –8 and 4.0×10 –8 M, respectively. Using this method, the levels of D ‐Asp and D ‐Glu in rat brain and human cerebrospinal fluid (CSF) were determined.