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Quantification of domoic acid in shellfish tissues by pressurized capillary electrochromatography
Author(s) -
Wu Weimin,
Wu Xiaoping,
Lin Xucong,
Xie Zenghong,
Giesy John P.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200900017
Subject(s) - domoic acid , chromatography , capillary electrochromatography , shellfish , shellfish poisoning , chemistry , matrix (chemical analysis) , electrochromatography , capillary action , capillary electrophoresis , fish <actinopterygii> , materials science , toxin , fishery , aquatic animal , biochemistry , composite material , biology
A method was developed to quantify domoic acid (DA), the chemical responsible for amnesic shellfish poisoning (ASP), by pressurized CEC (pCEC). The effect of different experimental conditions on the separation of DA and matrix solutes, such as the content of ACN in mobile phase, pH and concentration of buffer, supplementary pressure and applied voltage, were investigated. Under the optimal conditions, the pCEC method separated DA from shellfish matrices within 6 min. By using supplementary pressure, bubble formation in the capillary column was completely suppressed. The method was repeatable, sufficient accurate and sensitive for rapid screening of DA in shell seafood.