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Hybrid silica polymeric monolith‐based in‐tube microextraction and CE for determination of bisphenol A in beverages
Author(s) -
Hu Jiwei,
Li Xuegang,
Cai Yawen,
Han Heyou
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800751
Subject(s) - monolith , chromatography , monolithic hplc column , chemistry , capillary electrophoresis , detection limit , capillary action , bisphenol a , electrokinetic phenomena , theoretical plate , extraction (chemistry) , analytical chemistry (journal) , materials science , high performance liquid chromatography , catalysis , biochemistry , organic chemistry , epoxy , composite material
On‐line concentration techniques in capillary electrophoresis, including stacking and sweeping, have a weak tolerance towards complex real samples. A phenyl‐functionalized hybrid silica monolithic polymeric capillary column (id 530 μm) was prepared by in‐situ co‐condensation of phenyltriethoxysilane (PTES) and tetraethoxysilane (TEOS). In order to enhance the stability of a monolithic column within a wide‐bore capillary, a novel and simple frit‐preparation method for wide‐bore capillaries was first introduced by immobilizing a short length of capillary of suitable outer diameter at the end of the monolithic capillary column during condensation of the sol‐gel solution. The monolithic column was used for the extraction of bisphenol A (BPA) from cola samples, and the interfering compounds can be removed from the samples with 40% ACN solution (v/v) as eluent. The pretreated samples were further concentrated and separated using large‐volume stacking and sweeping micellar electrokinetic chromatography. The method was successfully applied to the determination of bisphenol A spiked in cola, yielding a detection limit of 1.8 ng/mL. Close correlation coefficients and acceptable method reproducibility were obtained for bisphenol A over a linear range of 0.005–0.200 μg/mL.