Fast analysis of prominent flavonoids in tomato using a monolithic column and isocratic HPLC | Zendy

Biesaga Magdalena | Zendy; Ochnik Urszula | Zendy; Pyrzynska Krystyna | Zendy

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Fast analysis of prominent flavonoids in tomato using a monolithic column and isocratic HPLC

Author(s) -

Biesaga Magdalena,

Ochnik Urszula,

Pyrzynska Krystyna

Publication year - 2009

Publication title -

journal of separation science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.72

H-Index - 102

eISSN - 1615-9314

pISSN - 1615-9306

DOI - 10.1002/jssc.200800730

Subject(s) - myricetin , naringin , chromatography , rutin , kaempferol , chemistry , quercetin , high performance liquid chromatography , detection limit , elution , organic chemistry , antioxidant

RP HPLC–UV–MS was optimized for simultaneous determination of several prominent flavonoids (quercetin, naringenin, naringin, myricetin, rutin and kaempferol) using a commercially available monolithic column and isocratic elution. The concentration of organic modifier (methanol and ACN) was tested in order to obtain the best resolution. The LODs for a UV detector at 254 nm were comprised between 0.1 μg/mL for myricetin and quercetin and 0.5 μg/mL for rutin, naringin and kaempferol with the precision in the range of 1.5–3.0% RSD. MS detection with multiply reaction monitoring (MRM) offers a considerable gain in selectivity as well as sensitivity LODs for flavonoids were in the range of 1–20 ng/mL. The developed method was used for monitoring the content of some flavonoids in tomatoes during their maturation process.

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