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Separation of structural, geometrical and optical isomers of epoxycarotenoids using triacontyl‐bonded stationary phases
Author(s) -
MeléndezMartínez Antonio J.,
EscuderoGilete Maria L.,
Vicario Isabel M.,
Heredia Francisco J.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800717
Subject(s) - chromophore , epoxide , structural isomer , elution , cis–trans isomerism , chemistry , molecule , chromatography , organic chemistry , catalysis
Abstract The efficiency of C 30 stationary phases in the separation of carotenes and diverse hydroxycarotenoids has been the subject of several studies. However, little is known concerning their ability to resolve epoxycarotenoids isomers, whose study is of great importance due to the functions they serve and the information they can reveal concerning the processing of foods. We have concluded that C 30 columns provide an excellent separation of structural, geometrical and optical isomers of epoxycarotenoids and that the presence of 5,8‐epoxide groups leads to a better shape recognition, to the extent that over 10 geometrical–optical isomers of 5,8‐epoxycarotenoids have been separated. Additionally, it was observed that these carotenoids elute later than their 5,6‐epoxide counterparts, albeit the latter have a longer chromophore.