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Temperature and pH‐stability of commercial stationary phases
Author(s) -
Teutenberg Thorsten,
Hollebekkers Koen,
Wiese Steffen,
Boergers Andrea
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800712
Subject(s) - polybutadiene , packed bed , column chromatography , divinylbenzene , analytical chemistry (journal) , methanol , chemistry , polystyrene , chromatography , zirconium , materials science , styrene , copolymer , polymer , inorganic chemistry , organic chemistry
In this paper, the temperature and pH stability of silica‐based RP stationary phases were investigated. Furthermore, nonsiliceous phases like a polymeric column based on polystyrene divinylbenzene and a polybutadiene coated zirconium dioxide column were also included. The columns were heated up to 150°C at dynamic conditions, which means that the eluent consisting of water and methanol (90:10, v/v) was continuously purged through the packed bed. After every 5 h, the columns were cooled down to room temperature and the efficiency was measured by injecting a test sample based on the Neue test. It could be shown that some stationary phases exhibited a very good temperature stability at the test conditions specified above.